[Objective] Melia azedarach is a multi-purpose fast growing timber tree species widely distributed in China. It is important to study the geographic variation pattern for seedlots and adaptive regions selection. [Method] 12 key traits of stone and seeds of 70 M. azedarach provenances collected from 17 provinces were used to analyze the geographic variation patterns by trend surface method.[Result] The results reveal that the traits variation of stones and seeds from different provenance are controlled by both longitude and latitude, but mainly the latitude variation. The performance within the distribution area in China totally follows a continuity of cline pattern. Among the traits observed, the width of stone and seeds shows a southwest-northeast gradient variation while hundred grain weight of stone and seeds mainly presents a northwest-southeast gradient variation. And peel 2 of stone shows a trend of south-north gradient variation. A positive linear relationship or a reverse parabolic curve relationship was found between traits and seedlots elevation, the width and weight of seeds and stones increased by the seedlot elevations. Based on the stone and seed traits, the cluster analysis and principal component analysis, the provenances can be divided into four groups.[Conclusion] Morphological differences of Melia Azedarach provenance shows that along with the change of the geographical factors, a variety of provenance forms their own ecological traits. Under similar geographical environment, the provenance traits performs similar trend. Increasing the molecular genetic evidence and long-term multi-location provenance trial could validate the reliability of the provenance regionalization.

[目的]借鉴以往楝属文献报道的SSR引物,筛选高度多态性、稳定性高、重复性好的苦楝SSR引物,为苦楝遗传图谱构建、QTL定位和分子标记辅助选择育种等研究领域应用奠定基础.[方法]本研究采用单因素法和正交试验设计进行SSR-PCR反应体系优化,并利用该体系以8个不同种源的苦楝基因组DNA为模板,从135对候选SSR引物中进行引物筛选.[结果]苦楝SSR-PCR最优反应体系为:1.0 μL 50 ng·μL-1模板DNA,1.2 μL 100 μmol·L-1引物,1.0 μL 10 mmol·L-1 dNTPs,0.8 μL 25 mmol·L-1 Mg2+,0.15 μL 5 U·μL-1 Taq酶,1.5 μL 10×Buffer,补ddH2O至15 μL;最终筛选出15对具有高度多态性、稳定性高、重复性好的SSR引物.[结论]本研究成功优化了苦楝SSR-PCR反应体系,并成功筛选出15对适用于苦楝的SSR引物.

目的 优化相关序列扩增多态性(SRAP)体系内的不同组分，建立适用于红椿SRAP分子标记的反应体系，并进一步从SRAP引物组合中筛选出稳定、多态性好的引物组合，为红椿遗传多样性研究奠定试验基础。 方法 针对SRAP-PCR反应体系中5个因素各设置8个水平，先利用单因素试验确定浓度梯度，后在确定的梯度范围内选定4个水平，按照正交试验L₁₆(4⁵)进行优化，结合正交直观分析法和新复极差法对各因素进行优化筛选。 结果 确定最优体系为总体系25 μL，模板DNA 25 ng，上下游引物各0.3 μmol·L^-1，Taq DNA聚合酶1U，Mg²⁺ 2.5 mmol·L^-1，dNTP 0.3 mmol·L^-1。利用稳定的SRAP-PCR体系，从1 505对SRAP引物组合中筛选出30对优质引物组合。 结论 通过不同种源红椿基因组DNA的重复验证，获得了稳定清晰、多态性较强的扩增条带，表明所确定的最优体系稳定可靠，适用性较强，可用于不同种源红椿遗传多样性研究的后续实验。

木豆(Cajanus cajan(L.)Millsp.)系南亚热带分布较广的具有较高经济价值的木本植物。国外对这一树种有专门的研究所进行综合性研究。国内这一树种属半野生状态,品种杂乱,多年来主要用于放养紫胶虫。近年来随着饲料工业的兴起,木豆作为木本蛋白饲料来源已进行了一些开发研究。